The Wang lab is interested in elucidating the catalytic mechanisms and structural basis of heme enzymes involved in natural product biosynthesis. These enzymes catalyze remarkable transformations, yet their molecular mechanisms remain poorly understood. This seminar will highlight three such systems: PrnB in pyrrolnitrin biosynthesis, Bmp7 in biaryl coupling, and RufO in peptide nitration. An overview of our research program will be presented, emphasizing an integrated approach that combines spectroscopic and structural methods. Through these studies, we characterize the heme center, probe enzyme-ligand interactions, capture reactive intermediates, and define enzymatic functions. Collectively, our work expands the understanding of heme chemistry and provides new insights into nature’s strategies for biocatalysis.