Advances in Extraction and Analysis of Daidzein for Therapeutic Applications

Daidzein, a bioactive isoflavonoid found in soybeans, roselle, and other legumes, has gained attention for its antioxidant, anti-inflammatory, and estrogenic properties.¹ Its therapeutic potential in bone health, cardiovascular protection, and cancer prevention has positioned it as a key compound in nutraceuticals and functional foods.^1,2  Accurate extraction and analysis of daidzein are critical for quality control, pharmacokinetics studies, and broader applications in the growing market for plant-based bioactives.^2,3  Traditional extraction methods such as solvent extraction, liquid-liquid extraction, and solid-phase extraction have been effective but often face challenges such as high solvent use, matrix interference, and reproducibility issues.^3,4 Recent advancements, including microwave-assisted extraction, ultrasound-assisted extraction, supercritical fluid extraction, and pressurized liquid extraction, offer faster, greener, and more efficient alternatives.⁵ These emerging techniques continue to play a pivotal role in quantifying and characterizing daidzein, enabling deeper insights into its structure and bioavailability. However, challenges persist in achieving high selectivity, scalability, and reproducibility in complex plant matrices. This presentation provides a comprehensive overview of conventional and emerging methodologies for the extraction and analysis of daidzein, highlighting key advancements and their implications for pharmacological and nutraceutical applications. 

References 

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2. Zhang H, Liu S, Li H, Xue F, Han S, Wang L, Cheng Y, Wang X. Extraction of isoflavones from Puerariae lobata using subcritical water. RSC advances. 2018;8(40):22652-8. 

3. Kazlauskaite JA, Ivanauskas L, Bernatoniene J. Novel Extraction Method Using Excipients to Enhance Yield of Genistein and Daidzein in Trifolium pratensis L. Pharmaceutics. 2021 May 22;13(6):777. 

4. Nan G, Gao Y, Guo L, Meng X, Yang G. Solid-liquid extraction of daidzein and genistein from soybean: Kinetic modeling of influential factors. Preparative Biochemistry and Biotechnology. 2018 Nov 26;48(10):946-53. 

5. Gao E, Wang W, Huang Y, Luo Z, Chen B, Xiao S, Li D. Puerariae lobatae Radix: Progress in extraction, separation methods and pharmacological activities research. Separations. 2024 Jun 24;11(7):195.