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Direct Mass Spectrometry Technologies for Intraoperative Tissue Analysis and Surgical Margin Evaluation

Portrait of Mehrnoush Taherzadeh, graduate student speaker
Mehrnoush Taherzadeh
Graduate Student, Department of Chemistry
University of Georgia
iSTEM Building 2, Room 1218
Analytical Seminar

Surgical resection remains a primary treatment for solid tumors. However, achieving tumor-free margins during surgery is challenging. Residual cancer cells at surgical margins are associated with increased recurrence and reduced survival rates. Intraoperative evaluation of surgical margins could enable more complete resection and reduce positive margins. Traditional frozen section histopathology has limitations including time-consumption, sampling errors, and availability of expertise. Therefore, there is need for new rapid intraoperative technologies to analyze surgical margins. Direct analysis of tissue using mass spectrometry (MS) is emerging as a promising approach. MS can provide molecular profiles of surgical specimens within minutes. Different MS technologies have been applied for intraoperative tissue identification and classification, including Desorption Electrospray Ionization (DESI) MS1, Rapid Evaporative Ionization (REIMS)2, and MS Pen3. These tools show high accuracy for detecting cancerous tissues and defining surgical margins. Additional developments in MS technology, data analysis algorithms, and workflow could enable routine adoption of MS-guided surgery in the future. Ultimately, this has potential to improve surgical outcomes and reduce positive margins for cancer patients.

 (1) Margulis K.; Chiou A. S.; Aasi S. Z.; Tibshirani R. G.; Tang J. Y.; Zare R. N. Distinguishing malignant from benign microscopic skin lesions using desorption electrospray ionization mass spectrometry imaging. PNAS 2018, 115, 6347–6352.

 (2) Van HeseL.; De Vleeschouwer S.; Theys T.; Larivi`ere E.; Solie L.; Sciot R.; Siegel T. P.; Rex S.; Heeren R. M.; Cuypers E. Towards real-time intraoperative tissue interrogation for REIMS-guided glioma surgery. JMSACL  2022, 24, 80–89.

 (3) Keating M.; Zhang J.; Feider C. L.; Retailleau S.; Reid R.; Antaris A.; Hart B.; Tan G.; Milner T. E.; Miller K.; Eberlin L. S. Integrating the MasSpec Pen to the da Vinci Surgical System for In Vivo Tissue Analysis during a Robotic Assisted Porcine Surgery. Anal. Chem. 2020, 92, 11535−11542.

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