Associate Professor

Contact Info

Office:
671
Phone Number:
Lab Office:
674, 675
Other Affiliations:
Education:
  • Ph.D., University of Nebraska-Lincoln
  • Postdoc, University of Wisconsin-Madison / University of Illinois Urbana-Champaign
Research Areas:
Research Interests:
  • Protein structure and function
  • NMR spectroscopy of proteins and protein complexes
  • Biophysical characterization of proteins
  • Structural biology

The molecular details that define the structure, stability and function of interesting proteins and protein complexes, and those that dictate how proteins fold and interact with one another, are the focus of the research in the Urbauer laboratory. We employ an approach combining modern biophysical and molecular biology techniques. Advanced biomolecular NMR spectroscopy is emphasized, and is used to analyze the structures of proteins at high resolution and to study their dynamics and stabilities. For example, we have used NMR to solve the structure of a dimeric regulator of prokaryotic transcription regulation, and are currently using site-directed mutagenesis along with analytical ultracentrifugation, fluorescence spectroscopy, and other biophysical measurements to characterize comprehensively the contributions of individual amino acids to the stability and reactivity of the protein. Our core projects include the study of gene regulation and novel regulators of transcription initiation in bacteria, calcium signaling mediated by the quintessential and ubiquitous calcium signaling protein calmodulin, and nuclear hormone receptor activation. These projects are important fundamentally, and they are relevant for biomedical concerns including antibiotic target development, oxidative stress and biological aging, and diseases such as breast cancer.

Selected Publications:

2018

Wand, A. J., Ehrhardt, M. R., & Urbauer, J. L. (2002). US6362624 B1, Apparatus and Method for High Pressure NMR Spectroscopy.

Urbauer, J. L. (2000). Transcription regulation by AsiA:  Solution structure of AsiA and elucidation of the AsiA-sigma-70 interface. In Biochemistry and Biophysics Section Faculty Seminar Series. Lawrence, Kansas: The Department of Molecular Biosciences (The University of Kansas).

Urbauer, J. (2004). Structure, stability and function of the anti-sigma factor AsiA and the AsiA-sigma 70 complex. In invited seminar. Department of Biophysics (University of Central Florida).

Urbauer, J. L. (2001). Structure and stability of the anti-sigma factor AsiA. In FASEB Summer Research Conference. Saxton's River, Vermont: Federation of American Societies for Experimental Biology (FASEB).

Urbauer, J. L., Cowley, A. B., Niedermaier, H. T., Broussard, H. E., & Urbauer, R. J. B. (2016). Structure and Physical Studies of the AlgH protein from Pseudomonas aeruginosa and the AlgH Protein Family. Retrieved from http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000387152400289&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=bccb20a1f2d949998a887c45eb30dd98

Urbauer, J. L. (2002). Structure and function of the anti-sigma factor AsiA. In 16th Annual Symposium of the Protein Society. San Diego, CA: The Protein Society.

Urbauer, J. L. (1998). Structure and dynamics of AsiA and the AsiA-sigma-70 complex. In Young Investigator Research Seminars. Philadelphia, Pennsylvania: Department of Biochemistry and Biophysics and Johnson Research Foundation (The University of Pennsylvania School of Medicine).

Urbauer, J. L. (2002). Solution structure and function of the anti-sigma factor AsiA. In invited seminar. Kansas City, Kansas: The Department of Biochemistry and Molecular Biology (The University of Kansas Medical Center).

Urbauer, J. L. (2001). Sigmas, anti-sigmas and antibiotics:  Structural biology of transcription regulation by the anti-sigma factor AsiA. In Higuchi Biosciences Center Fall Sciences Talks. The University of Kansas, Lawrence, Kansas: Higuchi Biosciences Center (The University of Kansas).

Urbauer, J. L. (2003). Regulation of transcription by the anti-sigma factor AsiA. In FASEB Summer Research Conference. FASEB (Federation of American Societies for Experimental Biology).

Urbauer, J. L. (2003). Regulation of target protein action through oxidative modification. In Workshop on Proteomic Approaches to Oxidative Stress and Biological Aging. Pacific Northwest National Laboratories (Richland, Washington).

Urbauer, J. (2005). Regulating prokaryotic transcription by the novel anti-sigma factor AsiA: structure and function of AsiA and AsiA-polymerase interactions. In invited seminar. Baton Rouge, LA: Department of Biological Sciences (Louisiana State University).

Urbauer, J. L. (1998). Recent studies of calmodulin and calmodulin complexes with calmodulin binding domains. In invited seminar. Gainesville, Florida: Interdisciplinary Center for Biotechnology Research (The University of Florida).

Urbauer, J. (2004). Prokaryotic transcription regulation by the novel anti-sigma factor AsiA. In invited seminar. Department of Chemistry (Georgia State University).

Urbauer, J. L. (2003). Practical aspects of protein structure determination using NMR. In invited seminar. Centers of Biomedical Research Excellence (The University of Kansas).

Niedermaier, H., Jolly, C., Bieber Urbauer, R., & Urbauer, J. (n.d.). NMR Studies of Estrogen Receptor Alpha Activation by Calmodulin. In 2011 Southeast Regional Meeting of the American Chemical Society (SERMACS). Richmond, VA: American Chemical Soceity.

Urbauer, J. (2016). New mechanistic insights into prokaryotic transcription regulation by the anti-sigma factor AsiA. In invited seminar. Department of Molecular and Cellular Biochemistry (The Ohio State University).

Urbauer, J. L. (2003). New insights into prokaryotic transcription regulation and the function of the anti-sigma factor AsiA. In invited seminar. The Department of Chemistry and The Department of Biochemistry and Molecular Biology (The University of Georgia).

Urbauer, J. L. (1999). Molecular recognition by calmodulin:  Structure, energetics and dynamics of calmodulin-peptide interactions. In invited seminar. Kansas City, Missouri: School of Biological Sciences (University of Missouri-Kansas City).

Urbauer, J. L. (1999). Interactions of calmodulin with calmodulin-binding domains:  Structure, dynamics and energetics. In invited seminar. Lawrence, Kansas: The Department of Molecular Biosciences (The University of Kansas).

Joshua, R., Urbauer, J. L., & Wicker, L. (n.d.). Characterization of separated citrus PME isozyme through heat stability, salt dependency, proteomics and action pattern. Journal Agricultural and Food Chemistry / American Chemical Society.

Urbauer, J. L. (1999). Calmodulin:  Old dog, new tricks. In Friday Research Discussions Seminar Series. Department of Biochemistry and Biophysics (The University of Pennsylvania School of Medicine).

Urbauer, J. L. (2000). Calmodulin and its interactions with calmodulin binding proteins:  NMR studies of structure, energetics and dynamics. In invited seminar. Manhattan, Kansas: The Department of Biochemistry (The Kansas State University).

Cowley, A. B., Urbauer, R. J. B., & Urbauer, J. L. (2005). 1H, 13C and 15N NMR Assignments for AlgH, a Putative Transcriptional Regulator from Pseudomonas Aeruginosa. Unknown Journal, 33, 74. doi:10.1007/s10858-005-1271-9

2017

Xia, T. ; Sriram, N. ; Lee, S. A. ; Altman, R. ; Urbauer, J. L. ; Altman, E. ; Eiteman, M. A. Glucose consumption in carbohydrate mixtures by phosphotransferase-system mutants of Escherichia coli. Microbiology 2017, 163, 866 - 877.

2015

Xia, T. ; Han, Q. ; Costanzo, W. V. ; Zhu, Y. ; Urbauer, J. L. ; Eiteman, M. A. Accumulation of d-glucose from pentoses by metabolically engineered Escherichia coli. Appl Environ Microbiol 2015, 81, 3387-94.

Urbauer, J. L. ; Cowley, A. B. ; Broussard, H. P. ; Niedermaier, H. T. ; Urbauer, R. J. Bieber Solution structure and properties of AlgH from Pseudomonas aeruginosa. Proteins 2015, 83, 1137-50.

Lübker, C. ; Urbauer, R. J. Bieber; Moskovitz, J. ; Dove, S. ; Weisemann, J. ; Fedorova, M. ; Urbauer, J. L. ; Seifert, R. Membranous adenylyl cyclase 1 activation is regulated by oxidation of N- and C-terminal methionine residues in calmodulin. Biochem Pharmacol 2015, 93, 196-209.

Lübker, C. ; Dove, S. ; Tang, W. - J. ; Urbauer, R. J. Bieber; Moskovitz, J. ; Urbauer, J. L. ; Seifert, R. Different Roles of N-Terminal and C-Terminal Domains in Calmodulin for Activation of Bacillus anthracis Edema Factor. Toxins (Basel) 2015, 7, 2598-614.

2014

Rhine, M. A. ; Rodrigues, A. V. ; Urbauer, R. J. Bieber; Urbauer, J. L. ; Stemmler, T. L. ; Harrop, T. C. Proton-induced reactivity of NO⁻ from a {CoNO}⁸ complex. J Am Chem Soc 2014, 136, 12560-3.

2010

Zhang, R. ; Hua, G. ; Urbauer, J. L. ; Adang, M. J. Synergistic and Inhibitory Effects of Aminopeptidase Peptides on Bacillus thuringiensis Cry11Ba Toxicity in the Mosquito Anopheles gambiae. Biochemistry 2010.

Gilmore, J. M. ; Bieber Urbauer, R. J. ; Minakhin, L. ; Akoyev, V. ; Zolkiewski, M. ; Severinov, K. ; Urbauer, J. L. Determinants of affinity and activity of the anti-sigma factor AsiA. Biochemistry 2010, 49, 6143-54.

2008

Lee, H. ; Rivner, J. ; Urbauer, J. L. ; Gartil, N. ; Wicker, L. De-esterification pattern of Valencia orange pectinmethylesterases and characterization of modified pectins. Journal of the Science of Food and Agriculture 2008, 88, 2102-2110.

2007

Slaughter, B. D. ; Urbauer, R. J. ; Urbauer, J. L. ; Johnson, C. K. Mechanism of calmodulin recognition of the binding domain of isoform 1b of the plasma membrane Ca(2+)-ATPase: kinetic pathway and effects of methionine oxidation. Biochemistry 2007, 46, 4045-54.

2006

Nemec, K. N. ; Pande, A. H. ; Qin, S. ; Urbauer, R. J. B. ; Tan, S. H. ; Moe, D. ; Tatulian, S. A. Structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A(2). Biochemistry 2006, 45, 12448-12460.

Xiong, Y. ; Chen, B. ; Smallwood, H. S. ; Urbauer, R. J. ; Markille, L. M. ; Galeva, N. ; Williams, T. D. ; Squier, T. C. High-affinity and cooperative binding of oxidized calmodulin by methionine sulfoxide reductase. Biochemistry 2006, 45, 14642-54.

2005

Urbauer, R. J. B. ; Gilmore, J. M. ; Rosasco, S. E. ; Hattle, J. M. ; Cowley, A. B. ; Urbauer, J. L. Cloning, high yield overexpression, purification, and characterization of AlgH, a regulator of alginate biosynthesis in Pseudomonas aeruginosa. Protein Expression and Purification 2005, 43, 57-64.

Cowley, A. B. ; Urbauer, R. J. ; Urbauer, J. L. 1H, 13C and 15N NMR assignments for AlgH, a putative transcriptional regulator from Pseudomonas aeruginosa. J Biomol NMR 2005, 33, 74.

Bieber Urbauer, R. J. ; Gilmore, J. M. ; Rosasco, S. E. ; Hattle, J. M. ; Cowley, A. B. ; Urbauer, J. L. Cloning, high yield overexpression, purification, and characterization of AlgH, a regulator of alginate biosynthesis in Pseudomonas aeruginosa. Protein Expr Purif 2005, 43, 57-64.

Slaughter, B. D. ; Unruh, J. R. ; Allen, M. W. ; Urbauer, R. J. B. ; Johnson, C. K. Conformational substates of calmodulin revealed by single-pair fluorescence resonance energy transfer: Influence of solution conditions and oxidative modification. Biochemistry 2005, 44, 3694-3707.

Anbanandam, A. ; Bieber Urbauer, R. J. ; Bartlett, R. K. ; Smallwood, H. S. ; Squier, T. C. ; Urbauer, J. L. Mediating molecular recognition by methionine oxidation: conformational switching by oxidation of methionine in the carboxyl-terminal domain of calmodulin. Biochemistry 2005, 44, 9486-96.

Yang, W. ; Wilkins, A. L. ; Ye, Y. ; Liu, Z. R. ; Li, S. Y. ; Urbauer, J. L. ; Hellinga, H. W. ; Kearney, A. ; van der Merwe, P. A. ; Yang, J. J. Design of a calcium-binding protein with desired structure in a cell adhesion molecule. J Am Chem Soc 2005, 127, 2085-93.

Slaughter, B. D. ; Unruh, J. R. ; Price, E. S. ; Huynh, J. L. ; Urbauer, R. J. B. ; Johnson, C. K. Sampling unfolding intermediates in calmodulin by single-molecule spectroscopy. Journal of the American Chemical Society 2005, 127, 12107-12114.

Osborn, K. D. ; Zaidi, A. ; Urbauer, R. J. B. ; Michaelis, M. L. ; Johnson, C. K. Single-molecule characterization of the dynamics of calmodulin bound to oxidatively modified plasma-membrane Ca2+-ATPase. Biochemistry 2005, 44, 11074-11081.

2004

Osborn, K. D. ; Zaidi, A. ; Mandal, A. ; Urbauer, R. J. ; Johnson, C. K. Single-molecule dynamics of the calcium-dependent activation of plasma-membrane Ca2+-ATPase by calmodulin. Biophysical Journal 2004, 87, 1892-9.

Osborn, K. D. ; Bartlett, R. K. ; Mandal, A. ; Zaidi, A. ; Urbauer, R. J. ; Urbauer, J. L. ; Galeva, N. ; Williams, T. D. ; Johnson, C. K. Single-molecule dynamics reveal an altered conformation for the autoinhibitory domain of plasma membrane Ca(2+)-ATPase bound to oxidatively modified calmodulin. Biochemistry 2004, 43, 12937-44.

Gregory, B. D. ; Nickels, B. E. ; Garrity, S. J. ; Severinova, E. ; Minakhin, L. ; Urbauer, R. J. ; Urbauer, J. L. ; Heyduk, T. ; Severinov, K. ; Hochschild, A. A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme. Proc Natl Acad Sci U S A 2004, 101, 4554-9.

Slaughter, B. D. ; Allen, M. W. ; Unruh, J. R. ; Urbauer, R. J. B. ; Johnson, C. K. Single-molecule resonance energy transfer and fluorescence correlation spectroscopy of calmodulin in solution. Journal of Physical Chemistry B 2004, 108, 10388-10397.

Allen, M. W. ; Urbauer, R. J. B. ; Johnson, C. K. Single-molecule assays of calmodulin target binding detected with a calmodulin energy-transfer construct. Analytical Chemistry 2004, 76, 3630-3637.

Zheng, K. ; Makagiansar, I. T. ; Wang, M. ; Urbauer, J. L. ; Kuczera, K. ; Siahaan, T. J. Expression, purification, and structural study of the EC4 domain of E-cadherin. Protein Expr Purif 2004, 33, 72-9.

Allen, M. W. ; Urbauer, R. J. ; Zaidi, A. ; Williams, T. D. ; Urbauer, J. L. ; Johnson, C. K. Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments. Anal Biochem 2004, 325, 273-84.

2003

Osborn, K. D. ; Singh, M. K. ; Urbauer, R. J. B. ; Johnson, C. K. Maximum-likelihood approach to single-molecule polarization modulation analysis. Chemphyschem 2003, 4, 1005-1011.

Bartlett, R. K. ; Bieber Urbauer, R. J. ; Anbanandam, A. ; Smallwood, H. S. ; Urbauer, J. L. ; Squier, T. C. Oxidation of Met144 and Met145 in calmodulin blocks calmodulin dependent activation of the plasma membrane Ca-ATPase. Biochemistry 2003, 42, 3231-8.

Wang, L. ; Bieber Urbauer, R. J. ; Urbauer, J. L. ; Benson, D. R. House fly cytochrome b5 exhibits kinetically trapped hemin and selectivity in hemin binding. Biochem Biophys Res Commun 2003, 305, 840-5.

Simeonov, M. F. ; Bieber Urbauer, R. J. ; Gilmore, J. M. ; Adelman, K. ; Brody, E. N. ; Niedziela-Majka, A. ; Minakhin, L. ; Heyduk, T. ; Urbauer, J. L. Characterization of the interactions between the bacteriophage T4 AsiA protein and RNA polymerase. Biochemistry 2003, 42, 7717-26.

Smallwood, H. S. ; Galeva, N. A. ; Bartlett, R. K. ; Urbauer, R. J. ; Williams, T. D. ; Urbauer, J. L. ; Squier, T. C. Selective nitration of Tyr99 in calmodulin as a marker of cellular conditions of oxidative stress. Chem Res Toxicol 2003, 16, 95-102.

Minakhin, L. ; Niedziela-Majka, A. ; Kuznedelov, K. ; Adelman, K. ; Urbauer, J. L. ; Heyduk, T. ; Severinov, K. Interaction of T4 AsiA with its target sites in the RNA polymerase sigma70 subunit leads to distinct and opposite effects on transcription. J Mol Biol 2003, 326, 679-90.

2002

Makagiansar, I. T. ; Nguyen, P. D. ; Ikesue, A. ; Kuczera, K. ; Dentler, W. ; Urbauer, J. L. ; Galeva, N. ; Alterman, M. ; Siahaan, T. J. Disulfide bond formation promotes the cis- and trans-dimerization of the E-cadherin-derived first repeat. J Biol Chem 2002, 277, 16002-10.

Tatulian, S. A. ; Urbauer, R. J. B. ; Smallwood, H. S. ; Bartlett, R. ; Urbauer, J. L. ; Squier, T. C. Oxidation of Met(144) and Met(145) in calmodulin blocks alpha-helix formation within the calmodulin-binding sequence of the plasma membrane Ca-ATPase normally associated with calmodulin binding and enzyme activation. Biophysical Journal 2002, 82, 57A-57A.

Urbauer, J. L. ; Simeonov, M. F. ; Urbauer, R. J. ; Adelman, K. ; Gilmore, J. M. ; Brody, E. N. Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions. Proc Natl Acad Sci U S A 2002, 99, 1831-5.

Makagiansar, I. T. ; Ikesue, A. ; Nguyen, P. D. ; Urbauer, J. L. ; Urbauer, R. J. ; Siahaan, T. J. Localized production of human E-cadherin-derived first repeat in Escherichia coli. Protein Expr Purif 2002, 26, 449-54.

2001

Urbauer, J. L. ; Adelman, K. ; Urbauer, R. J. ; Simeonov, M. F. ; Gilmore, J. M. ; Zolkiewski, M. ; Brody, E. N. Conserved regions 4.1 and 4.2 of sigma(70) constitute the recognition sites for the anti-sigma factor AsiA, and AsiA is a dimer free in solution. J Biol Chem 2001, 276, 41128-32.

Makagiansar, I. T. ; Nguyen, P. ; Ikesue, A. ; Urbauer, R. J. B. ; Urbauer, J. L. ; Siahaan, T. J. Disulfide bond promotes cis- and trans-dimeric formation of E-cadherin-derived EC1 and EC12 domains. Faseb Journal 2001, 15, A895-A895.

Flynn, P. F. ; Urbauer, R. J. B. ; Zhang, H. ; Lee, A. L. ; Wand, A. J. Main chain and side chain dynamics of a heme protein: N-15 and H-2 NMR relaxation studies of R. capsulatus ferrocytochrome c(2). Biochemistry 2001, 40, 6559-6569.

1999

Wilkinson, K. D. ; Laleli-Sahin, E. ; Urbauer, J. ; Larsen, C. N. ; Shih, G. H. ; Haas, A. L. ; Walsh, S. T. ; Wand, A. J. The binding site for UCH-L3 on ubiquitin: mutagenesis and NMR studies on the complex between ubiquitin and UCH-L3. J Mol Biol 1999, 291, 1067-77.

Skalicky, J. J. ; Gibney, B. R. ; Rabanal, F. ; Urbauer, R. J. B. ; Dutton, P. L. ; Wand, A. J. Solution structure of a designed four-alpha-helix bundle maquette scaffold. Journal of the American Chemical Society 1999, 121, 4941-4951.

Wand, A. J. ; Urbauer, J. L. ; Ehrhardt, M. R. ; Lee, A. L. NMR studies of protein-peptide complexes: Examples from the calmodulin system. In Peptide and Protein Drug Analysis; Reid, R. E. ; Peptide and Protein Drug Analysis; Marcel Dekker, Inc., 1999; Vol. 101, pp. 727-752.

1998

Urbauer, J. L. ; Bradshaw, D. E. ; Cleland, W. W. Synthesis of (2R,3R)-erythro- and (2R,3S)-threo-fluoromalate using malic dehydrogenase; stereoselectivity of malic dehydrogenase. Biochemistry 1998, 37, 18018-25.

Urbauer, J. L. ; Bradshaw, D. E. ; Cleland, W. W. Determination of the kinetic and chemical mechanism of malic enzyme using (2R,3R)-erythro-fluoromalate as a slow alternate substrate. Biochemistry 1998, 37, 18026-31.

1997

Urbauer, J. L. ; Adelman, K. ; Brody, E. N. Main-chain NMR assignments for AsiA. J Biomol NMR 1997, 10, 205-6.

Lee, A. L. ; Urbauer, J. L. ; Wand, A. J. Improved labeling strategy for 13C relaxation measurements of methyl groups in proteins. J Biomol NMR 1997, 9, 437-40.

Edens, W. A. ; Urbauer, J. L. ; Cleland, W. W. Determination of the chemical mechanism of malic enzyme by isotope effects. Biochemistry 1997, 36, 1141-7.

Wand, A. J. ; Urbauer, J. L. ; McEvoy, R. P. ; Bieber, R. J. Internal Dynamics of Human Ubiquitin Revealed by 13C-Relaxation Studies of Randomly Fractionally Labeled Protein. In Techniques in Protein Chemistry; Marshak, D. R. ; Techniques in Protein Chemistry; Academic Press, 1997; pp. 715-725.

1996

Urbauer, J. L. ; Ehrhardt, M. R. ; Bieber, R. J. ; Flynn, P. F. ; Wand, A. J. High-resolution triple-resonance NMR spectroscopy of a novel calmodulin peptide complex at kilobar pressures. Journal of the American Chemical Society 1996, 118, 11329-11330.

Wand, A. J. ; Urbauer, J. L. ; McEvoy, R. P. ; Bieber, R. J. Internal dynamics of human ubiquitin revealed by 13C-relaxation studies of randomly fractionally labeled protein. Biochemistry 1996, 35, 6116-25.

1995

Ehrhardt, M. R. ; Urbauer, J. L. ; Wand, A. J. The energetics and dynamics of molecular recognition by calmodulin. Biochemistry 1995, 34, 2731-8.

Urbauer, J. L. ; Short, J. H. ; Dow, L. K. ; Wand, A. J. Structural analysis of a novel interaction by calmodulin: high-affinity binding of a peptide in the absence of calcium. Biochemistry 1995, 34, 8099-109.

Wand, A. J. ; Bieber, R. J. ; Urbauer, J. L. ; McEvoy, R. P. ; Gan, Z. Carbon relaxation in randomly fractionally 13C-enriched proteins. J Magn Reson B 1995, 108, 173-5.

1994

Waldrop, G. L. ; Braxton, B. F. ; Urbauer, J. L. ; Cleland, W. W. ; Kiick, D. M. Secondary 18O and primary 13C isotope effects as a probe of transition-state structure for enzymatic decarboxylation of oxalacetate. Biochemistry 1994, 33, 5262-7.

Robertson, D. E. ; Farid, R. S. ; Moser, C. C. ; Urbauer, J. L. ; Mulholland, S. E. ; Pidikiti, R. ; Lear, J. D. ; Wand, A. J. ; DeGrado, W. F. ; Dutton, P. L. Design and synthesis of multi-haem proteins. Nature 1994, 368, 425-32.

Qi, P. X. ; Urbauer, J. L. ; Fuentes, E. J. ; Leopold, M. F. ; Wand, A. J. Structural water in oxidized and reduced horse heart cytochrome c. Nat Struct Biol 1994, 1, 378-82.

Leopold, M. F. ; Urbauer, J. L. ; Wand, A. J. Resonance assignment strategies for the analysis of NMR spectra of proteins. Mol Biotechnol 1994, 2, 61-93.

Fisher, P. J. ; Prendergast, F. G. ; Ehrhardt, M. R. ; Urbauer, J. L. ; Wand, A. J. ; Sedarous, S. S. ; McCormick, D. J. ; Buckley, P. J. Calmodulin interacts with amphiphilic peptides composed of all D-amino acids. Nature 1994, 368, 651-3.

1992

Janc, J. W. ; Urbauer, J. L. ; O'Leary, M. H. ; Cleland, W. W. Mechanistic studies of phosphoenolpyruvate carboxylase from Zea mays with (Z)- and (E)-3-fluorophosphoenolpyruvate as substrates. Biochemistry 1992, 31, 6432-40.

Waldrop, G. L. ; Urbauer, J. L. ; Cleland, W. W. N-15 Isotope Effects on Nonenzymatic and Aspartate-Transcarbamylase Catalyzed-Reactions of Carbamyl-Phosphate. Journal of the American Chemical Society 1992, 114, 5941-5945.

1991

Jones, J. P. ; Urbauer, J. L. Theoretical Kinetic Isotope Effects for the Hydride Transfer from Formate to Carbon-Dioxide - a Comparison of Theory with Experiment. Journal of Computational Chemistry 1991, 12, 1134-1141.

Weiss, P. M. ; Gavva, S. R. ; Harris, B. G. ; Urbauer, J. L. ; Cleland, W. W. ; Cook, P. F. Multiple isotope effects with alternative dinucleotide substrates as a probe of the malic enzyme reaction. Biochemistry 1991, 30, 5755-63.

1987

Urbauer, J. L. ; Dorgan, L. J. ; Tomaszek, T. A., J. ; Schuster, S. M. Dependence of the activity of beef heart mitochondrial adenosinetriphosphatase on the properties of the catalytic metal ion. Biochemistry 1987, 26, 2914-24.

1985

Gruys, K. J. ; Urbauer, J. L. ; Schuster, S. M. Metal-nucleotide structural characteristics during catalysis by beef heart mitochondrial F1. J Biol Chem 1985, 260, 6533-40.

1984

Dorgan, L. J. ; Urbauer, J. L. ; Schuster, S. M. Metal dependence and thermodynamic characteristics of the beef heart mitochondrial adenosine triphosphatase. J Biol Chem 1984, 259, 2816-21.

Urbauer, J. L. ; Dorgan, L. J. ; Schuster, S. M. Effects of deuterium on the kinetics of beef heart mitochondrial ATPase. Arch Biochem Biophys 1984, 231, 498-502.